http://localhost:80/xmlui/handle/123456789/43 2026-05-08T17:53:07Z http://localhost:80/xmlui/handle/123456789/343 Title: Cross sectional study on the association among hypertension with obesity indicators and dietary patterns of fishing community at coastal regions in India Authors: Alam, Seikh Sahanawaz Abstract: Background and aim: One of the main factors of sickness and disability around the world is hypertension and obesity is the main predictor of hypertensive condition. A few population-based studies on fishermen in West Bengal, India, evaluated the association between anthropometric parameters, nutritional intake, and obesity indicators with blood pressure (BP). The present investigation aims to explore the association between obesity indicators and nutrient consumption with blood pressure. Methods: In the present study, a number of 1202 male fishermen (ages 25–60 years) from West Bengal’s coastal areas, took their anthropometric measurements, BP values, and nutritional intake using standard techniques. The cut-off values of body mass index (BMI) and percentage of body fat (BF%) correlated with hypertension were determined by using receiver operating characteristic analysis. The statistical analyses were erformed using SPSS (Version 20) and MedCalc statistical software (version 20.115). Results: The study showed that most of the anthropometric parameters were higher in the control group than the fishermen, and most of the fishermen remain in normotensive conditions. Obesity indicators were significantly high in hypertensive individuals. Increasing of the obesity indicators were associated with hypertensive condition. In the case of nutrient intake, only fat and oil intake had a significant positive correlation with an increase in BP. This finding also suggests that the BMI and BF% cut off values were associated with hypertension. Conclusion: This study suggests that the increasing value of BMI and the cut-off value of BMI and BF% of this study are all potential risk factors for hypertension. 2024-05-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/342 Title: Prevalence and molecular detection of tick borne pathogens in goats and ticks from different parts of North Eastern regions of India Authors: Alam, Seikh Sahanawaz Abstract: Despite the fact that the climate of North-East (NE) India is suitable for tick diversity, no systematic study has been done regarding the prevalence of ticks and tick-borne pathogens affecting small ruminants. A total of 1053 goats belonging to different age groups, breeds, and sex were examined from April 2019 to March 2020. Blood smear examination and PCR assays were conducted to detect tick-borne pathogens in the collected samples. The tick species recorded were Rhipicephalus (Boophilus) microplus, Hyalomma anatolicum anatolicum, and Haemaphysalis bispinosa. The overall prevalence of tick-borne pathogens was 32.28%. Mixed infection with Theileria sp. and Anaplasma sp. was most common followed by single infections of Anaplasma sp. and Theileria sp. A significantly higher rate of infection was observed in female animals. Species-specific PCR revealed different tickborne pathogens like Anaplasma marginale, Anaplasma centrale, and Theileria luwenshuni in goats. Isolated DNA samples of ticks were found to be positive for A. marginale, A. centrale, and T. luwenshuni and Coxiella burnetii in three genera of ticks with PCR assay. The results showed that vector-borne intracellular haemoprotozoa and Anaplasma are prevalent in the study area in apparently healthy small ruminants and the identified ticks have an endosymbiotic relationship with C. burnetii. 2022-02-16T00:00:00Z http://localhost:80/xmlui/handle/123456789/341 Title: Molecular detection of Eimeria acervulina isolated from broiler birds Authors: Alam, Seikh Sahanawaz Abstract: The present study reports the histopathological, haematological and biochemical changes in broiler chicken naturally infected with Eimeria acervulina. A polymerase chain reaction (PCR) was used to identify this species in 225 gut samples of broiler chicken from different farms in North Eastern (NE) region of India. Postmortem examination revealed greyish white transeversely elongated area on the serous surface, oedema, together with necrosis and sloughing of intestinal epithelium.Haematological changes included a decrease in haemoglobin,(Hb) and packed cell volume. The value of mean corpuscular haemoglobin concentration (MCHC), on the otherhand increased slightly. Biochemical changes showed a significant increase in the level of glucose, cholesterol, Alaninine amino transferase (ALT), Asparase amino transferase (AST) and Alkaline phosphatase. The PCR assay was based on internal transcribed spacer (ITS1) region of the ribosomal DNA ofEimeria sp which has shown interspecies variation that enables to differentiate the species. The six isolates of Eimeria acervulina obtained were sequenced and a phylogenetic tree wasprepared. The sequences of the six isolates were searched for matching with isolates available in the Gen Bank database for sequence similarity using nucleotide blast. The sequence analysis showed that the newly isolates of E. acervulina had 99% Similarity with isolate of Turkey origin. 2021-01-01T00:00:00Z http://localhost:80/xmlui/handle/123456789/340 Title: Investigation of tick-borne pathogens by conventional and molecular techniques in cattleof North-Eastern region of India Authors: Alam, Seikh Sahanawaz Abstract: The present study underscores the investigation on prevalence of tick-borne pathogens of cattle both from hosts as well vectors from this part of India by conventional blood smear examination and through molecular techniques. A total of 1,153 cattle belonging to different age groups, breeds, sex were screened during the period from April, 2020 to March, 2021. Blood smear examination as well as PCR assay were followed to detect tick-borne pathogens in collected samples. The tick species recorded were Rhipicephalus (Boophilus) microplus, Hyalomma anatolicum anatolicum and Haemaphysalis leachi leachi .Overall prevalence of tick-borne pathogens was recorded as 33.30%. Mixed infection with Theileria sp. and Anaplasma sp. was found highest among infected cattle followed by single infection of Anaplasma sp. And Theileria sp. A few samples were only found to be positive for Babesia sp. infection. Significantly higher rate of infection was observed in the female animals. Species-specific PCR revealed different tick-borne pathogens species viz. Babesia bigemina, Theileria orientalis, Theileria annulata, Anaplasma marginale, Anaplasma central and Anaplasma bovis in cattle. Isolated DNA samples from blood and ticks were found positive for Coxiella burnetii and Borrelia burgdorferi sensu lato by PCR assay, albeit at very low percentage. The results clearly indicate that vector-borne haemoprotozoa and rickettsia are prevalent in the study area in apparently healthy animals without showing any obvious symptoms and R.(B.)microplus tick has an endosymbiotic relationship with C. burnetii and B. burgdorferi sensu lato. 2021-08-01T00:00:00Z